Skip to main content

Translate

Lipemia may produce significant interference in the analytical results of different biochemical parameters.



Lipemia is the increased concentration of triglyceride-rich lipoproteins in blood resulting in the cloudy/turbid appearance of serum or plasma. Lipemia in a blood specimen used for clinical evaluation can cause significant interference with obtaining accurate test values. Lipemia creates turbidity of a sample and is a result of the accumulation of lipid particles (mainly due to the presence of chylomicrons). These large particles create light scatter, resulting in elevated absorbance levels that impact colorimetric determinations. Many clinical analytes are found in the aqueous layer of plasma/serum, thus decreasing certain test values in lipemic specimens, while lipid-soluble analytes may be erroneously elevated. 
Lipemia causes clinically significant interferences for level of phosphorus, creatinine, total protein, calcium etc. 

Mechanism of Interference

  • Light scattering effects may increase absorbances during end point reactions and non-blanked reactions for some analytes.
  • Volume displacement effect greatly decreases the value of some analytes particularly electrolytes; Na+,K+.
  • Hemolysis of RBCs is enhanced in the presence of lipemia.
  • Non homogeneity of the sample.
  • Physical and chemical interferences.
Labels:

Comments

Post a Comment

Popular posts from this blog

The Pitfalls of Routine Health check-ups During Acute Illness: What You Need to Know

Preventive health check-ups , which typically include a series of blood tests, play a crucial role in maintaining overall well-being. These check-ups help identify potential health issues early, allowing for timely intervention. However, while these screenings are essential, the timing of when you undergo them is equally important. Getting routine blood tests during an acute illness—such as the flu, a stomach bug, or even a common cold—can lead to misleading results and unnecessary concerns. Here’s why it’s best to delay preventive health check-ups until after you've fully recovered from an acute illness. Understanding Acute Illness and Its Impact on Blood Tests: An acute illness refers to a sudden onset condition that usually lasts for a short period. Common examples include the flu, fever, a cold, or gastroenteritis (Vomiting, Diarrhoea). Although these illnesses are temporary, they cause significant changes in the body’s physiology as it works to fight off the infection or infla...
Post a Comment
Read more

High Urine Sugar and Normal Blood Sugar: Uncovering the Mysteries of Glycosuria

  Glycosuria , the presence of glucose in urine, is commonly associated with diabetes, where high blood sugar levels lead to the spillage of glucose into the urine. However, a perplexing scenario arises when glycosuria occurs alongside normal blood sugar levels. This intriguing condition challenges conventional medical understanding and prompts a deeper exploration into its causes and implications. Under normal circumstances, the kidneys filter blood, reabsorbing glucose back into the bloodstream and preventing its excretion in urine. Glycosuria typically indicates a disruption in this process, often linked to hyperglycemia (high blood sugar levels). But when blood glucose levels are within normal ranges, the puzzle deepens. Possible Causes of Glycosuria with Normal Blood Sugar:  Several conditions and factors can lead to this unusual phenomenon: Renal Glycosuria: A rare inherited condition where the renal tubules are unable to reabsorb glucose efficiently, leading to its pre...
Post a Comment
Read more

What is Human Metapneumovirus (hMPV)? Signs, Risks, and How to Stay Safe.

Human metapneumovirus (hMPV) is an important but often underappreciated respiratory virus that belongs to the Paramyxoviridae family. First identified in 2001, hMPV has emerged as a leading cause of acute respiratory infections (ARIs), particularly in young children, the elderly, and immunocompromised individuals. hMPV is distributed globally, with infections occurring year-round but peaking in late winter and early spring in temperate climates. Studies suggest that nearly all children are exposed to the virus by the age of five. Reinfections are common throughout life, although they tend to be less severe in healthy adults. Clinical Features:  The spectrum of hMPV infection ranges from asymptomatic to severe disease. Common clinical manifestations include: Mild upper respiratory tract symptoms: Runny nose, cough, and sore throat. Lower respiratory tract infections (LRTIs): Bronchiolitis, pneumonia, and exacerbation of asthma or chronic obstructive pulmo...
Post a Comment
Read more